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Basophilia, defined as more than 100 basophils/μL in venous blood, can be seen in various nonneoplastic and neoplastic conditions including chronic myeloproliferative neoplasms such as chronic myeloid leukemia (CML), polycythemia vera, or myelofibrosis.ĬML is a clonal stem cell disorder characterized by three phases, namely initial indolent chronic phase (CP) followed by an accelerated phase (AP), blast phase, or both. Available from: īasophils are the least common type of granulocyte representing 0.5%–1% of circulating white blood cells (WBCs) playing an important role in immune functions.
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Comparison of basophil count by Beckman Coulter UniCel DxH 800, Sysmex XN-1000, and manual microscopy in cases of suspected chronic myeloid leukemia.
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How to cite this URL: Chopra P, Bhardwaj S, Arora A. How to cite this article: Chopra P, Bhardwaj S, Arora A. Keywords: Automation, basophil, chronic myeloid leukemia All smears should be manually counted for basophils in cases of suspected CML because of its importance in clinical management. However, BC from none of the analyzers can be used alone without consideration of the microscopic results. In the 5%–10% range of BC, XN-1000 identified 59/78 cases whereas DxH 800 identified only 43/78 cases.ĬONCLUSION: With lower BC, Sysmex XN-1000 and, at higher BC, Beckman Coulter DxH 800 showed better performance. In addition, in the 10%–20% range of BC, XN-1000 identified 6/22 cases whereas DxH 800 identified 12/22 cases. In the frequency distribution analysis, XN-1000 missed all 10 cases with BC >20% whereas DxH 800 missed 3/10 cases with BC >20%. Bland–Altman plots showed a mean bias of 2.2% and 2.4% by XN-1000 and DxH 800, respectively, when compared with manual counts. Results of microscopically counted basophils correlated well among two pathologists ( r 2 = 0.92). BC obtained among both analyzers did not correlate ( r 2 = 0.14). RESULTS: The age of the patients ranged from 4 to 89 years, with a male-to-female ratio of 1.2:1 (148 males 121 females).
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They were compared with BC given by automated counters using correlation analysis and Bland Altman plots. The mean of basophil counts obtained microscopically was considered to be standard. MATERIALS AND METHODS: Two hundred and sixty-nine ethylenediaminetetraacetic acid samples identified as suspected CML run on Beckman Coulter UniCel DxH 800 and Sysmex XN-1000 were analyzed for BC microscopically on Giemsa-stained peripheral smear slides by two pathologists. In this study, we aim to compare the BC in patients with suspected CML using two automated analyzers with manual microscopy. Analysis of peripheral blood picture therefore holds its importance in these cases. Basophil counts (BCs) given by automated hematology analyzers are often not reliable. It helps differentiate patients of CML from those with leukemoid reaction. INTRODUCTION: Basophilia can help stratify cases of chronic myeloid leukemia (CML) into different phases and monitor response to therapy and has a significant prognostic value.